Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/5564
Title: | Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via down regulation of IFN-γ and IL-17 |
Authors: | Xue, Yuan Yang, Yong Su, Zhaoliang Barnie, Prince Amoatt Zheng, Dong Zhang, Yun Xu, Yan Wang, Shengjun Xu, Huaxi |
Issue Date: | 2013 |
Publisher: | University of Cape Coast |
Abstract: | Abstract.Th1 and Th17 cells are involved in the pathogenesis of rheumatoid arthritis (RA). T-bet, a Th1-specifc transcription factor, appears to drive the maturation of Th1 and IFN-γ secretion. In the present study, we established the T-bet shRNA recombinant plasmid (p-T-shRNA) and explored its possible anti-inflammatory effect in a collagen-induced arthritis (CIA) model by local injection of plasmid vectors. For the initiation of CIA, DBA/1J mice were immunized with type II collagen (CII) in Freund's adjuvant and the CII-immunized mice were treated with p-T-shRNA. Levels of T-bet, IFN-γ, IL-17 and RORγt mRNA in splenocytes and synovial joints were measured by quantitative real-time PCR and T-bet expression in joint tissue was detected by immunohistochemistry staining. The intracellular IFN-γ and IL-17 were analyzed by flow cytometry (FCM). The results demonstrated that therapeutic administration on the local joints with p-T-shRNA significantly suppressed IFN-γ and IL-17 gene expression and improved the pathogenesis of arthritis in CIA mice, while administration of a plasmid expressing T-bet (pIRES-T-bet) accelerated the disease onset. Our study suggests that T-bet may be developed as a potential target for arthritis therapy |
Description: | 5p:, ill. |
URI: | http://hdl.handle.net/123456789/5564 |
ISSN: | 23105496 |
Appears in Collections: | Department of Biomedical & Forensic Sciences |
Files in This Item:
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Local delivery of T-bet shRNA reduces.pdf | Article | 789.34 kB | Adobe PDF | View/Open |
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