Investigation of adsorption characteristics of fibrinogen on modified gold substrates using infrared reflection absorption spectroscopy

Abstract

The adsorption characteristics of human fibrinogen (HFG) on surfaces with well-controlled chemistries have been studied using infrared reflection absorption spectroscopy. The surfaces examined in this study provide the experimental basis for exploring fundamental non-covalent intermolecular forces that dominate protein adsorption processes. Comparisons were drawn between fibrinogen and non-specifically adsorbed bovine IgG (bIgG) as well as structurally rigid lysozyme (LYS) on a positively-charged amine-modified surface to further understand substrate-influence on protein surface coverage. Work presented herein shows that surface coverage of an adsorbed protein depends largely on the nature of the substrate and the protein structure