Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/8752
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dc.contributor.authorXei, Wei-
dc.contributor.authorLi, Daojuan-
dc.contributor.authorZhang, Juan-
dc.contributor.authorLi, Zhike-
dc.contributor.authorAcheampong, Desmond Omane-
dc.contributor.authorHe, Yuan-
dc.contributor.authorWang, Youfu-
dc.contributor.authorChen, Zhiguo-
dc.contributor.authorWang, Min-
dc.date.accessioned2023-09-30T09:05:59Z-
dc.date.available2023-09-30T09:05:59Z-
dc.date.issued2014-05-10-
dc.identifier.urihttp://hdl.handle.net/123456789/8752-
dc.description.abstractVEGF and its receptors, especially VEGFR2 (KDR), are known to play a critical role in angiogen- esis under both physiological and pathological conditions, including cancer and angiogenic retinopathies. This study was aimed at developing a fully human IgG1 antibody (mAb-04) constructed from a phage-derived scFv, target- ing the VEGF/VEGFR2 pathway. Firstly, an innovative transfection system, containing two recombinant expres- sion vectors (pMH3 and pCApuro), were introduced into CHO-s cells and clones with higher yield selected accord- ingly. After an optimal fermentation condition was deter- mined, fed-batch fermentation was performed in 5-L bioreactor with a final yield up to 60 mg/L. Further, cell proliferation, wound healing, transwell invasion, tube for- mation and chick embryo chorioallantoic membrane assays showed significant anti-angiogenic activity of mAb-04 in vitro and in vivo. In addition, the results of Western blot- ting indicated the ability of mAb-04 to inhibit VEGF- induced VEGFR2 signaling pathway. Finally, ADCC assay demonstrated that mAb-04 is capable of mediating tumor cell killing in presence of effector cells. This study has therefore proved that the full-length antibody targeting human VEGFR2 has potential clinical applications in the treatment of cancer and other diseases where pathological angiogenesis is involved.en_US
dc.language.isoenen_US
dc.publisherUniversity of Cape Coasten_US
dc.subjectVEGFR2 (KDR)en_US
dc.subjectHuman monoclonal antibodyen_US
dc.subjectCHO cell lineen_US
dc.subjectAnti-angiogenesisen_US
dc.subjectADCCen_US
dc.titleGeneration and characterization of a novel human IgG1 antibody against vascular endothelial growth factor receptor 2en_US
dc.typeArticleen_US
Appears in Collections:School of Allied Health Sciences

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