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http://hdl.handle.net/123456789/8752
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DC Field | Value | Language |
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dc.contributor.author | Xei, Wei | - |
dc.contributor.author | Li, Daojuan | - |
dc.contributor.author | Zhang, Juan | - |
dc.contributor.author | Li, Zhike | - |
dc.contributor.author | Acheampong, Desmond Omane | - |
dc.contributor.author | He, Yuan | - |
dc.contributor.author | Wang, Youfu | - |
dc.contributor.author | Chen, Zhiguo | - |
dc.contributor.author | Wang, Min | - |
dc.date.accessioned | 2023-09-30T09:05:59Z | - |
dc.date.available | 2023-09-30T09:05:59Z | - |
dc.date.issued | 2014-05-10 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/8752 | - |
dc.description.abstract | VEGF and its receptors, especially VEGFR2 (KDR), are known to play a critical role in angiogen- esis under both physiological and pathological conditions, including cancer and angiogenic retinopathies. This study was aimed at developing a fully human IgG1 antibody (mAb-04) constructed from a phage-derived scFv, target- ing the VEGF/VEGFR2 pathway. Firstly, an innovative transfection system, containing two recombinant expres- sion vectors (pMH3 and pCApuro), were introduced into CHO-s cells and clones with higher yield selected accord- ingly. After an optimal fermentation condition was deter- mined, fed-batch fermentation was performed in 5-L bioreactor with a final yield up to 60 mg/L. Further, cell proliferation, wound healing, transwell invasion, tube for- mation and chick embryo chorioallantoic membrane assays showed significant anti-angiogenic activity of mAb-04 in vitro and in vivo. In addition, the results of Western blot- ting indicated the ability of mAb-04 to inhibit VEGF- induced VEGFR2 signaling pathway. Finally, ADCC assay demonstrated that mAb-04 is capable of mediating tumor cell killing in presence of effector cells. This study has therefore proved that the full-length antibody targeting human VEGFR2 has potential clinical applications in the treatment of cancer and other diseases where pathological angiogenesis is involved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Cape Coast | en_US |
dc.subject | VEGFR2 (KDR) | en_US |
dc.subject | Human monoclonal antibody | en_US |
dc.subject | CHO cell line | en_US |
dc.subject | Anti-angiogenesis | en_US |
dc.subject | ADCC | en_US |
dc.title | Generation and characterization of a novel human IgG1 antibody against vascular endothelial growth factor receptor 2 | en_US |
dc.type | Article | en_US |
Appears in Collections: | School of Allied Health Sciences |
Files in This Item:
File | Description | Size | Format | |
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Generation and characterization of a novel human IgG1 antibody.pdf | Main Article | 2.69 MB | Adobe PDF | View/Open |
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