Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/8987
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dc.contributor.authorAcheampong, Desmond Omane-
dc.contributor.authorWang, Youfu-
dc.contributor.authorTang, Mingying-
dc.contributor.authorLiu, Fang-
dc.contributor.authorZhang, Juan-
dc.contributor.authorWang, Min-
dc.date.accessioned2023-10-03T19:32:01Z-
dc.date.available2023-10-03T19:32:01Z-
dc.date.issued2015-
dc.identifier.issn2277– 7105-
dc.identifier.urihttp://hdl.handle.net/123456789/8987-
dc.description.abstractThe use of antibody in targeted therapy has become the credible option in the treatment of cancers due to its specificity and the fact that it is associated with relatively lower toxicity compared to the other treatment options like chemotherapy and radiotherapy. However, Pharmaceutical 5N, 32N, 71N and 91N targeting vascular endothelial growth factor receptor 2 (VEGFR2) were generated from camel. The nanobodies University, Nanjingwere then screened by ELISA, immunoblotting and surface plasmon 210009, PR China.resonance (SPR) to select the nanobodies with high binding affinities. The nanobodies 5N and 32N demonstrated the highest binding affinities and therefore were selected for further studies. This was further confirmed by flow cytometry assay. Additionally, the selected nanobodies 5N and 32N demonstrated significant anti-angiogenic and anti-neoplastic abilities by restraining the proliferation of VEGFR2 expressing human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. These nanobodies are therefore potential anti-angiogenic agents which could possibly be used in cancer therapy.en_US
dc.language.isoenen_US
dc.subjectNanobody, .en_US
dc.subjectvascular endothial growth factor receptor 2en_US
dc.subjecttargeted therapyen_US
dc.subjectantibodyen_US
dc.titleEvaluation Of The Binding And Anti-Angiogenic Capacity Of Anti-Vascular Endothelial Growth Factor Receptor 2 Nanobody (Anti-Vegfr2 Nanobody)en_US
dc.typeArticleen_US
Appears in Collections:School of Allied Health Sciences



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