Design of a Comprehensive Biochemistry and Molecular Biology Experiment: Phase Variation Caused by Recombinational Regulation of Bacterial Gene Expression

Abstract

Scientific experiments are indispensable parts of Biochem- istry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about Sal- monella enterica serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation, antibody agglutination test, and PCR analysis. Phase variation was observed by baterial motility assay and identified by anti- body agglutination test and PCR analysis. This comprehen- sive experiment can be performed to help students improve their ability to use the knowledge acquired in C 2014 by The Inter- Biochemistry and Molecular Biology