Abstract:
This study examined the impact of incorporating roasted para rubber seed
kernel meal (RoPRSKM) into the feed of ICR albino mice, focusing on growth
performance, haematology, blood biochemistry and liver and kidney tissue
histology. In this study, four experimental diets, T1, which was the control made of
Koudjis feed (21% CP and 2825 kcalsME/kgDm) and T2, T3 and T4 in which ME
of RoPRSKM replaced 5%, 10 and 15%, respectively of T1 metabolizable energy
content were used. Using a completely randomized design, 40 male albino mice
were assigned to four dietary treatments. The proximate analysis of the RoPRSKM
indicated 24.8% crude protein, 9.2% crude fibre, 46.6% crude fat, 0.26 mg/kgDM
hydrogen cyanide (HCN), and estimated metabolisable energy of 4405.5 kcal
ME/kgDM. Inclusion of RoPRSKM in the meal showed no significant effect on the
weight gain and water intake of the albino mice. Feed intake significantly reduced
in mice feed on treatments T3 and T4. At the end of the experiment, Mice were
chosen randomly to collect blood for analysis of haematology and blood
biochemistry. Using terminal standard operating procedure, blood samples were
obtained from the orbit. To determine the packed cell volume, microhematocrit
centrifugation method, hemoglobin (Hb) was employed to measured
spectrophotometer, red blood cell (RBC), white blood cell and manually performed
using Merck Veterinary Manual.While mean corpuscular volume (MCV),mean
corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration
(MCHC), were calculated Blood parameters (red blood cells, white blood cells, etc.)
showed no significant detrimental effects in mice fed with RoPRSKM diets as
compared to the control group. In terms of blood biochemistry, the diets did not
exhibit any adverse effects across all the measured parameters, except for total
protein, Alkaline Phosphatase, and Globulin. These three parameters displayed
statistically significant differences among all the treatment groups. After the blood
samples collection, the mice were immediately euthanized humanely by cervical
dislocation. The abdomen was opened by an incision along the mid-ventral line and
the skin and musculature folded back to expose the internal organs. The liver and
kidney samples were harvested and fixed in 10% formalin solution for a week.
After that, Tissue samples were washed 24 hours under slow-speed running water,
dehydrated in graded concentrations of ethanol and immersed in ethanol - xylene,
xylene – paraffin1, paraffin wax I, paraffin wax II, paraffin wax III, and finally
embedded in paraffin wax. 5 μm thick slices were cut from each paraffin block
sample onto a glass slide, and stained with haematoxylin-eosin (HE) routinely.
Tissue structure was then observed under the Hund H600 microscope, and
photomicrographs were taken as images for presentation. However, the histology
examination revealed no observable alterations or changes in the liver and kidney
tissues of the mice when fed with RoPRSKM. The finding indicated that
incorporating RoPRSKM into the diet of ICR albino mice did not have significant
adverse effects on their growth, haematology, blood biochemistry, or liver and
kidney tissue structure. However, some variations were observed in certain
parameters, but were not significant as compared with the acceptable ranges.
